FIG. 5.

Typical chromatographic profiles of crude extracellular matrix of S. epidermidis RP62A grown under various conditions at 37°C on a Sephacryl S-300 column. Aliquots (200 μl) of each 5-ml fraction were assessed for neutral sugars (•) (at an optical density at 485 nm) and amino sugars (▪) (at an optical density at 530 nm). The data are data for 2 liters of each bacterial culture. Cells were grown with vigorous shaking in TSB (a) and BHI (b), under aero-anaerobic conditions with moderate shaking in TSB (c) and BHI (d), and as biofilms on glass surfaces without shaking in TSB (e) and BHI (f). OD, optical density; TSB_PS, polysaccharide fraction of TSB medium; BHI_PS, polysaccharide fraction of BHI medium.