FIG. 2.

NTHI outer membrane antigen induction of macrophage IL-1β. Macrophages were incubated with OMP P6, OMP P2, LOS, or the total OMP preparation, each at 0.1 μg/ml, and IL-1β induction was measured for 8, 24, and 48 h. Results shown in panel A are means ± SEM from triplicate samples of one donor and were reproducible with cells from three separate donors. Combined results of all three donors, shown in panel B, are expressed as IL-1β concentration elicited with each antigen relative to IL-1β elicited by total OMPs. Macrophage reactivity was confirmed by treatment with E. coli K235LPS (1 μg/ml) for each time point.