FIG. 1.
Size exclusion column elution patterns of Fr.IIS sulfuric acid hydrolysis products. Fr.IIS was treated with 0.6 N H2SO4 for 0 (A), 0.75 (B), 1.5 (C), 3 (D), 4.5 (E) and 6 (F) h at 100°C. At each time period, the treated samples were applied to a size exclusion column and eluted with distilled water, and carbohydrate content was monitored by the phenol-sulfuric acid method. M1, M2, and M3 indicate mono-, di-, and trisaccharides, respectively, removed from Fr.IIS by the sulfuric acid treatment. The amount of M1, M2, and M3 increased during the treatment, whereas the fractions eluted in the void volume (Vo) decreased with the same treatment. The elution pattern of scmn1/Fr.II treated with the sulfuric acid was essentially the same as that from hydrolysis of Fr.IIS (data not shown). O.D., optical density.