Contact-dependent cytotoxicity of levels of immortalized human MS-74 VECs by T. vaginalis. (A) Cytotoxicity results using different ratios of untreated and 20 mM DAB-treated parasites (columns C/D). The results were compared to the control, untreated (100/0, column C) and 20 mM DAB-treated (0/100, column D) organisms. The results are averages of three experiments, each with seven samples. (B) Results obtained with 20 mM DAB-treated parasites supplemented with millimolar amounts of putrescine (Put) and micromolar amounts of spermine (Sp) and spermidine (Sd). The numbers underneath the bars represent the amounts added. The cytotoxicity levels were compared to those of the control, untreated (column C) and 20 mM DAB-treated (column D) organisms, and the controls were normalized to 100% for comparison. The results are the average of three experiments, each with seven samples.