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One-dimensional substrate SDS-PAGE analysis of T. vaginalis T016 grown in the absence (lane C) or presence (lane DAB) of 20 mM DAB. Clear regions of the gel represent proteinase digestion of gelatin as substrate copolymerized with acrylamide. Arrows indicate two regions of proteinase activity shown to be involved in contact-dependent cytotoxicity (11, 12, 27).
