TABLE 3.

Amounts of bacteria and PCR amplification of 23S rRNA from mesenteric lymph nodes and spleens of mice infected with M. avium

Time point (mos after infection)a	Tissueb	Amt of bacteria (CFU/g)c	PCR amplification result
Before infection	Lymph node	Undetected	−
	Spleen	Undetected	−
1	Lymph node	26 ± 3	+
	Spleen	Undetected	−
2	Lymph node	32 ± 2	+
	Spleen	Undetected	−
4	Lymph node	132 ± 43	+
	Spleen	Undetected	+
6	Lymph node	313 ± 20	+
	Spleen	64 ± 6	+

^aUninfected mice were negative for M. avium 23S rRNA at all time points. Four animals were used for each time point. The mice were not treated with azithromycin.

^bSpleens weighed between 0.07 and 0.08 g.

^cThe lower limit of detection was 20 CFU per g of tissue.