TABLE 1.

Most significant genes upregulated by Act in human intestinal epithelial cells (HT-29) from 0 to 2 h as determined by four separate analysis methods (grouped by function)^a

GenBank no.	Gene name	Nonpolarized				Polarized, GCOS (FC)
		GCOS (FC)	SAM (FC)	Spotfire (FC)	ANOVA (P)
Apoptosis
BF594446	Myeloid cell leukemia sequence 1 (Bcl-2-related)	2.6	2.7	2.6	0.013611	5.3
NM_021960.1	Myeloid cell leukemia sequence 1 (Bcl-2-related)	2.5	2.5	2.3	0.018607	4
BF981280	Myeloid cell leukemia sequence 1 (Bcl-2-related)	2.4	3.2	3.0	0.001319	1.9
AI275690	Myeloid cell leukemia sequence 1 (Bcl-2-related)	2.1	2.4	2.3	0.014808	2.8
NM_007350.1	TDAG51	5.5	6.2	6.4	0.01555	3
AI795908	TDAG51	4.7	4.8	4.6	0.00381	4
AA576961	TDAG51	4.3	4.3	4.1	0.008432	4
NM_007350.1	TDAG51	2	2.2	2.2	0.007025	1.6
Cellular reorganization
AF099730	Gap junction protein, beta 3 (connexin 31)	2.3	2.1	2.1	7.66E-06	2.1
BF060667	Gap junction protein, beta 3 (connexin 31)	2.2	2.1	2.2	1.15E-06	2.3
Z19574	Cytokeratin 17	2.2	2.4	2.5	5.31E-20	NS
BG054844	Ras homolog gene family, member E (RhoE)	2.2	2.1	2.1	0.001669	1.6
Cell growth/Differentiation/Cell cycle     regulation
NM_000389.1	Cyclin-dependent kinase inhibitor 1A (p21, Cip1)	3.1	2.3	2.3	0.002638	3.2
NM_012242.1	Dickkopf homolog 1 (DKK1)	2.8	2.7	2.6	0.000874	NS
NM_001432.1	Epiregulin	2.7	2.9	2.8	0.012219	2.1
AF003934.1	Growth differentiation factor 15 (GDF15)	2.3	2.2	2.5	0.008893	NS
U77914.1	Jagged 1 (JAG1)	2.2	2.5	2.5	0.000286	1.7
U61276.1	Jagged 1 (JAG1)	2.1	2	2.1	6.33E-05	1.9
U73936.1	Jagged 1 (JAG1)	2.1	2.5	2.5	0.000304	1.7
NM_002467.1	Myc proto-oncogene protein (c-Myc)	2.1	2.3	2.2	0.013804	1.5
AF045451.1	NGFI-A binding protein 1 (Nab1)	3	3.3	3.3	0.015786	1.7
NM_002607.1	Platelet-derived growth factor alpha polypeptide	2.4	2.3	2.2	0.006248	1.5
AI263909	Ras homolog gene family, member B (RhoB)	2.7	2.9	2.8	0.001791	3.2
NM_023925.1	C1q domain containing 1	2.1	2	2.0	0.004791	2
Cytokines
NM_013246.1	Cardiotrophin-like cytokine; neurotrophin-1B-cell stimulating factor-3 (CLC)	2.5	2.3	2.6	0.00929	2.6
NM_001511.1	GRO1 oncogene	3.1	4.2	4.4	0.00368	NS
AF043337.1	IL-8	31.6	24.3	32.9	0.006866	1.9
NM_000584.1	IL-8	26.4	23.8	21.8	0.010181	3
NM_002309.2	Leukemia inhibitory factor (LIF)	2.5	2.7	2.8	0.007476	2.3
Extracellular matrix digestion
NM_002421.2	Matrix metalloproteinase 1 (MMP1)b	4	4.5	4.0	0.035324	2.6
AY029180.1	Soluble urokinase plasminogen activator receptor	2.1	2.4	2.3	0.006765	1.9
U08839.1	Urokinase-type plasminogen activator receptor	2	2	2.0	0.022107	1.7
Signal transduction
U16996.1	Dual specificity protein phosphatase 5	2.1	2	2.0	0.00587	4
NM_004431.1	Ephrin type-A receptor 2 (EphA2)	2.7	3.1	3.0	0.006323	2.6
U16797.1	Ephrin-B2	2.6	2.2	2.3	0.005556	2.8
BF001670	Ephrin-B2	2.1	2.1	2.2	0.006585	2.5
NM_002923.1	Regulator of G-protein signaling 2 (RGS2)	4.2	3.8	3.6	0.005268	2.8
Transcription factors
AL564683	CCAAT enhancer binding protein (CEBP), beta	2	2.1	2.0	0.029667	3.2
BG251266	Fra-1	5.2	5.3	5.9	0.001552	12.1
AL021977	MAFF-like protein	3.2	2.7	3.3	0.003684	1.7
NM_014755.1	SERTA domain containing 2	2	2	2.0	0.018674	2
Other/unknown
NM_025047.1	ADP-ribosylation factor 2 (cDNA, 79%)	2.3	2.6	2.5	0.000746	NSc
NM_000693.1	Aldehyde dehydrogenase 1 family, member A3	2.4	2.6	2.4	0.04679	2.1
NM_006033.1	Endothelial lipase	2.7	3.2	2.9	0.023085	1.6
NM_001450.1	LIM-protein 3	2.2	2.3	2.2	0.009135	1.6

^aFC, fold change. Genes listed twice were represented on the microarrays by more than one probe set, and each was determined separately to be significantly upregulated by Act.

^bAlso upregulated between 0 and 12 h, as determined by all four analysis techniques.

^cNS, no significant change.