TABLE 2.
Confirmation by real-time RT-PCR of selected genes determined to be upregulated by Act in human intestinal epithelial cells (HT-29) by using microarrays
| GenBank no. | Gene name | Fold changea
|
|||
|---|---|---|---|---|---|
| Nonpolarized
|
Polarized
|
||||
| Microarrays (0 vs 2 h) | RT-PCR (0 vs 2 h) | Microarrays (0 vs 2 h) | RT-PCR (0 vs 2 h) | ||
| NM_021960.1 | Mcl-1 | 2.3-2.5 | 2.4 | 1.9-5.3 | 2.8 |
| NM_007350.1 | TDAG51 | 5.5-6.4 | 16.7 | 1.6-4.0 | 7.8 |
| NM_000389.1 | Cip1 | 2.3-3.1 | 14.3 | 3.2 | 6.9 |
| NM_002467.1 | c-Myc | 2.1-2.3 | 2.4 | 1.5 | 2.1 |
| AF045451.1 | Nab1 | 3.0-3.3 | 2.7 | 1.7 | 2.5 |
| NM_013246.1 | Cardiotrophin-like cytokine | 2.3-2.6 | 5.4 | 2.6 | 4.2 |
| NM_001511.1 | GRO1 | 3.1-4.4 | 10.1 | NCa | NC |
| NM_000584.1 | IL-8 | 21.8-31.6 | 2.4 | 1.9-3.0 | 2.1 |
| NM_002309.2 | LIF | 2.5-2.8 | 14.6 | 2.3 | 2.9 |
| NM_002421.2 | MMP1 | 4.0-4.5 | 6.9 | 2.6 | 5.8 |
| NM_004431.1 | EphA2 | 2.7-3.1 | 11.5 | 2.6 | 3.9 |
| U16797.1 | Ephrin-B2 | 2.2-2.6 | 4.7 | 2.5-2.8 | 2.8 |
| NM_002923.1 | Rgs2 | 3.6-4.2 | 6.2 | 2.8 | 4.1 |
| BG251266 | Fra-1 | 5.2-5.9 | 2.9 | 12.1 | 7.5 |
a
NC, no change.