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. 2024 Feb 9;121(7):e2310479121. doi: 10.1073/pnas.2310479121

Fig. 1.

Fig. 1.

HIF stabilization is required for LD accumulation upon MYC inhibition in ccRCC cells. (A) Lipid droplet staining and (B) western blot of the indicated proteins in 786-O VHL+ and VHL− cells after MYC inhibition. (C) Lipid droplet staining in 786-O VHL+ EV, 786-O VHL+ Omomyc, 786-O VHL- EV, or 786-O VHL- Omomyc cells after vehicle (−Omomyc, H2O) or DOX treatment (+Omomyc). (D) Western blot of the indicated proteins in RCC4 VHL+ and VHL− cells after treatment with MYCis in 1% O2 (hypoxia). (E) Lipid droplet staining in RCC4 VHL+ and VHL− cells after treatment with MYCis in 1% O2 (hypoxia). (F) Lipid droplet staining in RCC4 VHL+ and RCC4 VHL− cells after treatment with MYCis in combination with DMSO (−chetomin) or chetomin. For western blots, β-actin was used as loading control. Molecular weight markers are shown to the Right. For fluorescence images: blue, DAPI; green, LD-BTD1 (LDs). (Scale bars: 20 μm.) All results are representative of three independent replicates.