Fig. 3.

Lipidomic analysis reveals different lipid species content in RCC4 VHL+ versus VHL- cells. (A) Mass isotopologue distribution of palmitate using U-¹³C₅-glutamine in RCC4 VHL+ and VHL− cells after treatment with JQ1. Data are presented as mean ± SD. Statistical analysis: two-way ANOVA with *, **, and **** indicating P < 0.05, <0.01, and <0.0001, respectively. (B) Heatmap with different lipid species in RCC4 VHL+ or VHL− cells after DMSO or JQ1 treatment, with red up-regulated and blue down-regulated lipid species. DG/DAG: diacylglycerides, PC: phosphatidylcholine, PC-O: phosphatidylcholine-O, PE: phosphatidylethanolamine, PE-O: phosphatidylethanolamine-O, LPG: lysophosphatidylglycerol, PG: phosphatidylglycerol, LPI: lysophosphatidylinositol, PI: phosphatidylinositol, LPS: lipopolysaccharide, PS: phosphatidylserine, TG: triglycerides, LPC: lysophosphatidylcholine, LPE: lysophosphatidylethanolamine, SL: saccharolipids, PA: phosphatidic acid. (C) Graphical representation of the different lipid species accumulated and the key enzymes in lipid metabolism upon JQ1 treatment in RCC4 VHL+ (purple) and VHL− (green). Image created with Biorender.com. G3P: glyceraldehyde 3-phosphate, FA-CoA: fatty acyl-CoA, LPAT: lysophosphatidyl acyltransferase, LPA: lysophosphatidic acid, A/GPAT: acyl/glycerol-3-phosphate acyltransferase, PAP: phosphatidic acid phosphatase, CDP-DAG: cytidine diphosphate-diacylglycerol, CDS: CDP-DAG synthase, PIS: phosphatidylinositol synthase, IP: inositol phosphate, DGAT: diacylglycerol acyltransferase. Lipidomic experiments were performed in three independent replicates.