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. 2024 Feb 5;121(7):e2310430121. doi: 10.1073/pnas.2310430121

Fig. 2.

Fig. 2.

YAP-MAML2 condensates contain transcriptional machinery and nascent RNA. (A) Fluorescence images of mEGFP-YAP-MAML2 and MAX-mKO3 in HEK293 cells. The arrows point to representative condensates. The fluorescence intensity profile (Right) is extracted from the position shown by the dashed line. Colocalization in example condensates is indicated by arrows. (B) Fluorescence images of YAP-MAML2 condensates with IF-imaged MED1. (C) Fluorescence images of YAP-MAML2 condensates with IF-imaged Pol II S5p. (D) Fluorescence images of YAP-MAML2 condensates with nascent RNA labeled by 5-ethynyluridine. (E) Percentage of YAP-MAML2 condensates that colocalize with puncta of other components. The percentage is determined by the ratio of coloca./YAP-MAML2 = number of colocalized condensates between YAP-MAML2 and TEAD4 divided by number of YAP-MAML2 condensates. Equivalent analysis for other pairs is also shown. Data are mean ± SD (n = 13 cells). [Scale bars, 5 μm (A–D).]