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. 2024 Feb 5;121(7):e2310430121. doi: 10.1073/pnas.2310430121

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Copyright © 2024 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 3. — YAP-MAML2 condensates dynamically disassemble and reassemble upon mitotic entry and exit. (A) Time-lapse images of HEK293 cells expressing mEGFP-YAP-MAML2 upon mitotic entry. The cells co-expressed monomeric infrared fluorescent protein (mIFP)-tagged H2B (in blue). Chromosome volume was calculated based on mIFP-H2B fluorescence. (Right) quantitative analysis of YAP-MAML2 condensate dissolution and chromosome condensation over time. Each line represents single cell traces (n = 6 cells). (B) Time-lapse images of HEK293 cells expressing mEGFP-YAP-MAML2 upon mitotic exit. (Right) quantitative analysis of YAP-MAML2 condensate reformation and chromosome de-condensation. Each line represents single cell traces (n = 6 cells). (Scale bars, 10 μm.)