Table 2.
Bioreactor systems, pH sensor types and run information used in this study.
| Bioreactor System Information |
Vessel Information | pH Sensor type | Bioreactor Identifier | Cell Line | Operation Type | Run Duration (days) | pH Setpoint | pH Control CO2 delivery |
|---|---|---|---|---|---|---|---|---|
| Benchtop Glass STR (Sartorius BioStat® B-DCUII) | Univessel® Glass 5 L | Autoclavable electrochemical probe | STR-1 | VRC01 | Fed-batch | 8 | 7.1 | CO2 sparging |
| STR-2 | VRC01 | Fed-batch | 8 | |||||
| STR-3 | TmAb | Perfusion | 13 | |||||
| STR-4 | TmAb | Perfusion | 20 | |||||
| Multi-parallel single-use STR (Sartorius Ambr® 250 Modular) | Modular Mammalian Vessel (001–2A23) | Single-use electrochemical probe | AMBR-1 | VRC01 | Fed-batch | 8 | 7.1 | CO2 sparging and overlay |
| AMBR-2 | VRC01 | Fed-batch | 8 | |||||
| AMBR-3 | VRC01 | Fed-batch | 8 | |||||
| AMBR-4 | VRC01 | Fed-batch | 5 | |||||
| AMBR-5 | VRC01 | Fed-batch | 11 | |||||
| AMBR-6 | VRC01 | Fed-batch | 11 | |||||
| AMBR-7 | VRC01 | Fed-batch | 11 | |||||
| AMBR-8 | VRC01 | Fed-batch | 11 | |||||
| AMBR-9 | VRC01 | Fed-batch | 10 | |||||
| AMBR-10 | VRC01 | Fed-batch | 10 | |||||
| AMBR-11 | VRC01 | Fed-batch | 10 | 7.3 | ||||
| AMBR-12 | VRC01 | Fed-batch | 10 | |||||
| Rocking bioreactor (Cytiva ReadyToProcess WAVE™ 25 rocker) | Cellbag BioClear 10, DOOPT II, pHOPT and screwcap (CB0002L10-33) | Single-Use optical sensor | WAVE-1 | VRC01 | Batch | 6 | 7.1 | CO2 overlay |
| WAVE-2 | VRC01 | Batch | 8 | |||||
| WAVE-3 | VRC01 | Fed-batch | 7 | |||||
| WAVE-4 | TmAb | Fed-batch | 14 | |||||
| Cellbag BioClear 10, DOOPT II, pHOPT and perfusion (CB0002L10-34) | WAVE-5 | VRC01 | Perfusion | 21 | ||||
| WAVE-6 | TmAb | Perfusion | 10 |