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Binding of M. pneumoniae total protein lysates to hSP-A. Radiolabeled mycoplasma total protein lysates, which were prepared in the presence of Ca2+ (open squares), Zn2+ (solid triangles), or Mg2+ (solid circles) were applied sequentially to control and hSP-A-coupled Sepharose columns. The NaCl gradient elution patterns of M. pneumoniae proteins which bound to hSP-A columns were generated by measuring radioactivity in 20-μl samples from 1-ml fractions.
