FIG. 6.

Inhibition of viable M. pneumoniae binding to immobilized hSP-A by antiserum to rMPN372. Microtiter wells were coated with 2 μg of hSP-A. [³⁵S]methionine-labeled M. pneumoniae cells were preincubated with serial dilutions of heat-inactivated anti-rMPN372 and prebleed sera (1:10, 1:100, and 1:1,000) for 1 h at room temperature. Bound rMPN372 was detected with anti-His tag monoclonal antibody and goat anti-mouse alkaline phosphatase-conjugated polyclonal antibodies (described in Fig. 5 legend and Materials and Methods), followed by development with p-nitrophenyl phosphate. Values represent the means and standard deviations of triplicate wells from three separate experiments. P ≤ 0.01 versus prebleed.