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. 2005 May;73(5):2991–2998. doi: 10.1128/IAI.73.5.2991-2998.2005

FIG. 1.

FIG. 1.

The dose of antigen required to restimulate T cells is reduced 10- to 20-fold by CyaA delivery. The numbers of IFN-γ-secreting SFC (IFN-γ SFC) were determined in an overnight ELISPOT in the presence of ESAT-6 or CFP-10 or their CyaA toxoid equivalents or mock CyaA toxoid containing no antigenic insert. The corrected response to antigen containing toxoid (i.e., minus the response to mock toxoid) ([CyaA-ESAT-6]-CyaA) is also shown. A) In nine healthy TST-positive responding donors, the recognition of recombinant ESAT-6 was optimal at 500 nM, whereas similar recognition occurred in the presence of 10-fold-less CyaA-ESAT-6. B) In 10 similar donors who responded to rCFP-10, CyaA delivery also shifted the dose-response curve to the left. Approximately 10 to 20 times less CFP-10 expressed as a CyaA-CFP-10 toxoid elicited the same response. Results are shown as means ± standard errors (error bars).