FIG. 2.
The detection of IFN-γ-secreting SFC (IFN-γ SFC) in low-responding subjects is enhanced by CyaA delivery. Subjects who responded to 500 nM rESAT-6 or 250 nM rCFP-10 were stratified by their magnitude of response into low (<50 IFN-γ-secreting SFC/106 PBMC), intermediate (50 to 100), and high (>100) responders. CyaA delivery significantly increased the detection of IFN-γ-secreting SFC in low-responding subjects. Average CyaA toxoid results are shown corrected for the background response to wild-type CyaA (CyaA-WT) ([CyaA-ESAT-6]-CyaA-WT). Results are shown as means ± standard errors of the means (error bars).