FIG. 3.

Both CD4⁺ and CD8⁺ responses can be detected by CyaA delivery. Immunomagnetic depletion of either CD4⁺ or CD8⁺ T cells from PBMC was performed, and the response of the remaining cells to CyaA toxoids was assayed. The response of CD4⁺-depleted PBMC was interpreted as CD8⁺ and vice versa. Both CD4⁺ and CD8⁺ responses to the toxoid antigens were seen, with the CD4⁺ response being dominant. Average responses are indicated by bars. IFN-γ SFC, IFN-γ-secreting SFC.