Table 1.
Ingredient composition of basal diet on as-fed basis and analyzed nutrient content on a dry matter basis of the basal diet in the direct amino acid oxidation study evaluating the minimum Phe requirement of adult cats (study 1)
| Ingredient | g kg−1 |
|---|---|
| Pregelatinized wheat starch1 | 287.00 |
| Water | 210.00 |
| Poultry fat | 135.00 |
| Amino acid premix2 | 134.00 |
| Lamb meal | 85.00 |
| Sucrose | 50.00 |
| Cellulose | 23.00 |
| Dicalcium phosphate | 15.00 |
| Sodium bicarbonate | 15.00 |
| Palatant3 | 13.00 |
| Brewer’s yeast | 10.00 |
| Potassium chloride | 8.00 |
| Calcium carbonate | 6.00 |
| Choline | 4.00 |
| NaCl | 3.00 |
| Vitamin premix4 | 1.00 |
| Mineral premix5 | 1.00 |
| Chemical composition | Analyzed content |
|---|---|
| Dry matter (DM), % | 76.77 |
| Metabolizable energy6, kcal kg−1 | 3224 |
| Crude Protein, % DM | 22.81 |
| Fat, % DM | 20.26 |
| Ash, % DM | 8.96 |
| Nitrogen-free extract, % DM | 47.97 |
| Amino acids, % DM | |
| Ala | 1.60 |
| Arg | 1.79 |
| Asp | 0.85 |
| Cys | 0.98 |
| Glu | 3.75 |
| Gly | 1.80 |
| His | 0.35 |
| Ile | 0.84 |
| Leu | 1.69 |
| Lys | 1.15 |
| Met | 0.33 |
| Met + Cys | 1.31 |
| Phe | 0.29 |
| Phe + Tyr | 2.17 |
| Pro | 1.44 |
| Ser | 1.03 |
| Thr | 0.65 |
| Trp | 0.32 |
| Tyr | 1.87 |
| Val | 0.85 |
1PAYGEL 290 Pregelatinized Wheat Starch, Archer Daniels Midland Company, USA.
2Amino acid premix contained per kilogram: 26.00 g L-glutamic acid, 16.00 g L-tyrosine, 10.00 g L-arginine and L-leucine, 8.00 g L-alanine, glycine and L-cysteine, 7.00 g proline, 6.00 g L-lysine, L-threonine, and L-serine, 5.00 g L-isoleucine, 4.00 g L-valine, taurine, and L-asparagine H2O e, 2.00 g DL-methionine, L-tryptophan, and L-histidine HCl H2O.
3PALASURANCE C45-140 Dry, Kemin Nutrisurance, Inc., USA.
4Vitamin composition of the premix: 105.83 mg/kg biotin, 1,573 mg/kg folic acid, 91,700 mg/kg niacin, 7,020 mg/kg pantothenic acid, 6,350 mg/kg pyridoxine, 4,930 mg/kg riboflavin, 10,100 mg/kg thiamin, 24.94 mg vitamin B12, 13,079,000 IU/kg vitamin A, 412,000 IU/kg vitamin D3, 46,718 IU/kg vitamin E.
5Mineral composition of the premix: 1.55% calcium, 0.0348% phosphorus, 0.4658 magnesium, 13.92% potassium, 0.2% sodium, 12.93% chloride, 11.90% sulfur, 15,493.9 ppm copper, 92,272.1 ppm iron, 9,397.4 ppm manganese, 361 ppm selenium, 104,105.8 ppm zinc, and 1,953 ppm iodine.
6Metabolizable energy = 3.5 kcal/g × crude protein (%) + 8.5 kcal/g × acid-hydrolyzed fat (%) + 3.5 kcal/g × nitrogen-free extract (%); nitrogen free extract (%) = 100% − (crude protein % + acid-hydrolyzed fat % + crude fiber % + ash %).