FIG. 4.

Sequence analysis of the region 5′ to the pMGA1.1 locus. Each sequence was derived from a single plasmid recombinant obtained by cloning PCR-amplified products from the indicated cloned mycoplasma cell lines. The cellular sources of the DNA templates used to obtain the cloned PCR products were normal cells (S6), cells grown in medium containing mAb66 [S6(mAb66)], and clones C11(+), C11(−), and C1. The numbers in brackets after the cell source indicate individual PCR clones. The sequence denoted pMGA1.1 is from reference 16 and was originally obtained by sequencing part of the M. gallisepticum S6 genome. Only nucleotides which differ from this (top) sequence are shown for the PCR clones, and dots indicate identities. Dashes indicate gaps in the alignment. The locations of the −10 and −35 promoter motifs (8) are indicated by solid lines. The last nucleotide (G) of each sequence represents the start point of transcription (+1). Sequences without asterisks were obtained by using as templates cloned, 6-kb PCR products. PCR clones with asterisks were from a separate experiment in which primers 1.1F and 1.1R (see Materials and Methods) were used to amplify only the region 5′ to pMGA1.1 (504 bp).