Extended data Figure 8 |. Multi-sequence alignment of mitochondrial ferredoxins.

The multi-sequence alignment was generated by Multalin⁴. Secondary structure elements of the human FDX2 structure are shown above the alignment according to PROMOTIF⁵. Numbering is according to the full-length sequences of human (Hs) FDX1 and FDX2 retrieved from Uniprot (https://www.uniprot.org). The Fe/S cluster-coordinating cysteine residues are highlighted in yellow. Altered residues/regions of human FDX1 and FDX2 are highlighted in grey (see also Suppl. Table S3). Residues/regions mutated for interconversion of FDX1 and FDX2 functions (mutants M3, M5, M6, M7, R135E and C-terminal deletion/exchange) are additionally highlighted by colored boxes for animal FDX1/2-type sequences. Names of organisms are colored according to FDX-type: animal FDX2 (red), mitosomal (black), plant (green), fungal (orange), animal FDX1 (blue) and bacterial (grey). Two Trypanosoma brucei (Tb) FDXs best align between fungal and FDX1-type (FdxA) proteins or between FDX1-type and bacterial (FdxB) proteins⁶. FDXs from the following organisms were used (sequence identifiers in brackets): Homo sapiens (NP_001026904.2, NP_004100.1), Canis lupus familiaris (XP_038284595.1, XP_038367022.1), Bos taurus (NP_001073695.1, NP_851354.1), Rattus norvegicus (NP_001101472.1, NP_058822.2), Mus musculus (NP_001034913.1, NP_032022.1), Danio rerio (NP_001070132.1, XP_001922722.2), Drosophila melanogaster (NP_001189075.1, NP_647889.2), Encephalitozoon cuniculi GB-M1 (NP_585988.1), Trachipleistophora hominis OX=72359 GN=THOM_0371 PE=4 SV=1 (L7K0F4_TRAHO), Arabidopsis thaliana (NP_001329852.1), Populus alba (XP_034922198.1), Oryza sativa Japonica Group (XP_015647182.1), Chaetomium thermophilum var. thermophilum DSM 1495 (XP_006692961.1), Chaetomium globosum CBS 148.51 (XP_001225251.1), Neurospora crassa OR74A (XP_958085.1), Aspergillus fumigatus Af293 (XP_747954.1), Yarrowia lipolytica CLIB122 (XP_500417.1), Saccharomyces cerevisiae S288C (NP_015071.1), Schizosaccharomyces pombe Etp1^fd (2WLB_A), Trypanosoma brucei brucei (XP_845713.1 and XP_844647.1), Magnetospirillum magnetotacticum (WP_009867486.1), Rhodospirillum rubrum (WP_200292119.1), Rickettsia prowazekii (WP_004595967.1), Yersinia pestis (WP_015683702.1), Shigella flexneri (EHF1065008.1), Salmonella enterica (WP_001124473.1).