Extended data Figure 2 |. Even high excess of FDX1 does not support enzymatic [2Fe-2S] cluster reconstitution on ISCU2.

Enzymatic synthesis of [2Fe-2S] clusters on ISCU2 in vitro was monitored by the CD signal at 431 nm for 10 min. Reactions included NIA, FXN, FDXR and the indicated equivalent amounts (eq.) of (a) FDX2 or (b) FDX1 relative to NIA. Representative experiments are shown and initial rates are presented in Fig. 1d.