Figure 1.

Small seeds are more dormant.

(A) Col-0 seed size distributions of four seed pools (n = 3802–5491).

(B) Size distribution of dry, freshly harvested Col-0 seeds (Supplemental Figure 1C). Mean values of seed sizes for dormant (dorm) and germinated (germ) seeds are shown as points for each of four replicates (n = 320–607). The p value of the Wilcoxon rank-sum test for comparison of seed sizes from all replicates is shown above the plot.

(C) Seed sizes and germination days of freshly harvested Col-0 seeds. The mean values of seed sizes are shown as points for each of the three replicates. The p value of the pairwise Wilcoxon rank-sum test (with false discovery rate correction) for the comparison of seed sizes from all replicates is shown above the plot.

(D and E) (D) The top 10% of the smallest and largest seeds were sorted, imaged, and tested (E) for germination after 7 days of secondary dormancy induction (n = 100). The p value of the two-sided paired t-test is shown above the plot. Seeds were sown and analyzed using a Boxeed robot. Seed germination was scored after 7 days.