Detection and analysis of YadA, O-ag, OC, and Ail expression in different Y. enterocolitica O:3 strains. (A) Immunoblot analysis using monoclonal antibodies specific for YadA (top), O-ag (middle), and OC (bottom). (B) Silver-stained SDS-PAGE gel showing outer membrane proteins isolated from Ail-expressing and -nonexpressing Y. enterocolitica O:3 and E. coli C600 strains. The arrows point to the migration level of the Ail band. The presence or absence of the factors is indicated below the panels.