FIG. 6.

C3 deposition and degradation on different Y. enterocolitica O:3 mutant bacteria. Bacteria were incubated in EGTA-Mg-treated serum and NHS for 3, 15, and 30 min. After being washed, C3 and its derivatives deposited on bacteria were identified by immunoblotting using monoclonal antibody specific for the α-chain of C3. Shown in frames are the depositions of the C3 derivatives on different types of bacteria at different time points. The bacteria were grown at 37°C unless otherwise indicated. For each bacterial sample, its phenotype with respect to expression of YadA, O-ag, Ail, and OC is given below the strain names. For each bacterial sample, C3 deposition in EGTA-Mg-treated serum (a) and in NHS (c) is indicated at the bottom of the figure.