TABLE 2.

Oligonucleotides used in this work

Name	Sequence (5′-3′)	5′-3′ position	Accession no.	Purpose
ail1	GACATTACTAGCTAGTTCTC	496-515	M29945	PCR
ail2	GAATCGATACCCTGCACCAAGC	1021-1000 (2731-2710)	M29945(AJ605740)	Deletion PCR
ail4	CAAGTAAGACGTCAATGGCATACGG	2592-2616	AJ605740	Deletion PCR
ail5	CGTTACTTCCCAGCCCCGGCAA	1859-1838	AJ605740	PCR
ail9	AAGCGCTTGAGGTCAAAGCACAA	1490-1512	AJ605740	PCR
ail10	TTAATGTGTACGCTGCGAGTGAA	2253-2275	AJ605740	PCR
trs19	AGCGTCGACGAAACCTTAAAAAC	1621-1640	Z47767	PCR
trs30	GAGCATTAACAGGAAAATAG	12935-12916	Z47767	PCR
trs47	CTGCAGACAAGCAAAAGGGCCAGGTGAA	1929-1908	Z47767	Deletion PCR, PstI site underlined
gale	CCTGG(C/T)GATAT(C/A)GCTGAGTGTTGGGC	12681-12706	Z47767	Deletion PCR
hindsph	AGCTCATG			HindIII-SphI adapter
MS16	CGCGGATCCATTAGGATTAATACAGGCGCA	2213-2196	X13882	yadA cloning, BamHI site underlined
MS17	CGCGGATCCTGCATGATTATCAGAAAAATA	478-495	X13882	yadA cloning, BamHI site underlined
MS20	CGCGAATTCATTAGGATTAATACAGGCGCA	2213-2196	X13882	yadA::KmGenBlock cloning, EcoRI site underlined
MS21	CGCGAATTCTGCATGATTATCAGAAAAATA	478-495	X13882	yadA::KmGenBlock cloning, EcoRI site underlined
MS27	TCGCTGCAGAATAAATAAATCCTGGTG	396-375	X06403	cat cloning, PstI site underlined
MS28	ATGCTGCAGCAATAGACATAAGCGGC	3952-3971	X06403	cat cloning, PstI site underlined