TABLE 3.
Complement resistance phenotypes and killing kinetics of Y. enterocolitica O:3 wt and mutant strains (grown at 37°C) in 66.7% NHS (based on data presented in Fig. 2 and 3)
| Phenotype (expressed factor)
|
Strain | Phenotype and kinetics at:
|
||||||
|---|---|---|---|---|---|---|---|---|
| 30 min
|
120 min
|
|||||||
| YadA | O-ag | Ail | OC | % Surviving | Killing kineticsa | % Surviving | Resistance phenotypeb | |
| YeO3-c-Ail-OCR | 0.13 ± 0.2 | Rapid | 0 ± 0 | Sensitive | ||||
| O-ag | YeO3-c-Ail-OC | 0.15 ± 0.3 | Rapid | 0.06 ± 0.2 | Sensitive | |||
| Ail | YeO3-c-OCR | 61.08 ± 18.2 | Delayed | 18.21 ± 8.9 | Intermediate | |||
| Ail | YeO3-028-OCR | 26.35 ± 5.5 | Rapid | 10.97 ± 7.8 | Intermediate | |||
| Ail | YeO3-c-trs8-R | 64.04 ± 7.6 | Delayed | 15.69 ± 6.7 | Intermediate | |||
| OC | YeO3-c-Ail-R | 8.1 ± 3.7 | Rapid | 0 | Sensitive | |||
| YadA | YeO3-Ail-OCR | 7.19 ± 1.6 | Rapid | 1.35 ± 0.7 | Intermediate | |||
| O-ag | Ail | YeO3-c-trs8 | 25.08 ± 10.6 | Rapid | 1.2 ± 1.1 | Intermediate | ||
| O-ag | Ail | YeO3-c-OC | 7.61 ± 3 | Rapid | 0.25 ± 0.6 | Sensitive | ||
| O-ag | Ail | YeO3-028-OC | 15.99 ± 6.3 | Rapid | 0.05 ± 0.1 | Sensitive | ||
| O-ag | OC | YeO3-c-Ail | 0 | Rapid | 0.02 ± 0.04 | Sensitive | ||
| Ail | OC | YeO3-028-R | 31.18 ± 9 | Delayed | 0.67 ± 0.6 | Sensitive | ||
| Ail | OC | YeO3-R | 88.93 ± 4.9 | Delayed | 15.94 ± 5.7 | Intermediate | ||
| YadA | O-ag | YeO3-Ail-OC | 15.04 ± 0.6 | Rapid | 4.46 ± 3.7 | Intermediate | ||
| YadA | Ail | YeO3-OCR | 36.84 ± 17.5 | Delayed | 17.73 ± 8.9 | Intermediate | ||
| YadA | Ail | YeO3-trs11-R | 52.75 ± 8.6 | Delayed | 25.78 ± 9.3 | Resistant | ||
| YadA | OC | YeO3-Ail-R | 10.86 ± 2.3 | Rapid | 3.67 ± 2.4 | Intermediate | ||
| O-ag | Ail | OC | YeO3-c | 4.81 ± 5.1 | Rapid | 0 | Sensitive | |
| O-ag | Ail | OC | YeO3-028 | 0.11 ± 0.13 | Rapid | 0.03 ± 0.1 | Sensitive | |
| YadA | O-ag | Ail | YeO3-trs11 | 35.72 ± 18.4 | Delayed | 28.31 ± 15.7 | Resistant | |
| YadA | O-ag | Ail | YeO3-OC | 58.91 ± 12.3 | Delayed | 45.03 ± 16.7 | Resistant | |
| YadA | O-ag | OC | YeO3-Ail | 20.1 ± 9.4 | Rapid | 3.58 ± 2 | Intermediate | |
| YadA | Ail | OC | YeO3-R2 | 87.3 ± 10.1 | Delayed | 70.85 ± 19.7 | Resistant | |
| YadA | O-ag | Ail | OC | YeO3 | 33.67 ± 5.7 | Delayed | 11.49 ± 5.8 | Intermediate |
a
Rapid, rapidly killed (<30% viable bacteria after 30 min); delayed, delayed type of killing (>30% viable bacteria after 30 min).
b
Sensitive, <1% viable bacteria after 120 min; intermediate, 1 to 20% viable bacteria after 120 min; resistant, >20% viable bacteria after 120 min.