FIG. 4.

Detection of SP-D in the cornea by immunofluorescence microscopy. (A) Normal mouse cornea viewed by immunofluorescence microscopy (magnification, ×1,200) with (left) and without (right) exposure to rabbit anti-mouse SP-D antiserum (dilution, 1:100). (B) Another experiment in which sections of wild-type (left) and SP-D (−/−) knockout (right) mouse corneas were exposed to the same SP-D-specific antiserum (dilution, 1:100). SP-D was strongly expressed in the corneal epithelium of the wild-type mouse.