FIG. 5.
Subtraction of SP-D from human tear fluid with mannan-conjugated Sepharose. Whole human tear fluid (triangles), mannan-Sepharose adsorbed human tear fluid (squares), and recombinant human SP-D (circles) were titrated on mannan-coated wells in the presence of 5 mM Ca2+ (solid symbols) or 5 mM EDTA (open symbols). The dilution series commenced at 1 μg of recombinant human SP-D/ml and at a 1:10 dilution of the tear fluid samples. The ELISA was developed as described in the legend to Fig. 1. Whole tear fluid contained ∼2 to 5 μg of SP-D/ml, and this was depleted by adsorption with mannan-conjugated Sepharose.