FIG. 4.

Toll-like receptor mRNAs in selected adult mouse organs. CD14, MD-2, and Tlr mRNAs were amplified by nested RT-PCR (see Materials and Methods), and samples of amplified products were electrophoresed in 1.5% agarose gels and visualized by ethidium bromide staining. Products were amplified by using the primer sets noted from RNA from adult bone marrow (A), small intestine (B), stomach (C), and colon (D), with the lanes at the left containing a 100-bp DNA ladder. Note that all samples of whole-organ RNA support amplification of all sequences.