FIG. 5.

CD14, MD-2, and Tlr mRNAs in isolated mouse Paneth cells and villus enterocytes. As described in the legend to Fig. 4, MD-2, CD14, and Tlr mRNAs were amplified by nested RT-PCR (see Materials and Methods), and samples of amplified products were electrophoresed in 1.5% agarose gels and visualized by ethidium bromide staining. Products noted in the panels were amplified from single intact crypts (A), single intact villi (B), replicate pools containing five villus enterocytes (C), and replicate pools containing five Paneth cells (3) (D). (A and B) Products of replicate RT-PCR are shown in adjacent lanes; (C and D) replicate reactions are aligned vertically. Lanes at the left contain a 100-bp DNA ladder.