FIG. 2.
Identification of antigenically variant DsrA proteins. (A) Two identical SDS-PAGE gels were prepared under reducing conditions with total cellular proteins from the indicated H. ducreyi strains and subjected to Western blotting. Panel 1, DsrA expression is detected using polyclonal anti-rFL-DsrAI made to rDsrAI from strain 35000HP; panel 2, DsrA expression is detected with MAb 4.79 made to rDsrAI from strain 35000HP. (B) H. ducreyi strains were examined for surface expression of class I and II DsrA in a dot blot format.