FIG. 4.
Identification of two classes of OMPs. Nine identically loaded SDS-PAGE gels (panels 1, 3, 6 and 7, 15% PAGE; panel 9, 12.5% PAGE; panels 2 and 5, 12% PAGE; panels 4 and 8, 7.5% PAGE) were prepared under reducing conditions with total cellular proteins or crude LOS from the indicated H. ducreyi strains and subjected to Western blotting, except for gel 9. Crude LOS in gel 9 was prepared as described in Materials and Methods and subjected to silver staining after electrophoresis. The class of each strain is indicated at the bottom of the figure. N-terminal antibodies were made to the N-terminal two-thirds of each recombinant protein (Table 3 and Fig. 1). Arrows indicate strains that reacted weakly with the antisera against DsrAI protein.