FIG. 3.

Specificity of antibodies induced by subpatent infection, measured by IFA. Serum was collected from naive, patently infected, and subpatently infected mice prior to the challenge infection (Fig. 1A). Binding of serum IgG to homologous P. c. chabaudi AS (A) and heterologous P. c. chabaudi CB (B) acetone-fixed schizonts was assessed by immunofluorescent staining with Texas Red-conjugated anti-mouse IgG and Hoechst (n = 10). Representative images are shown. Similar results were obtained in two separate experiments.