FIG. 7.
A single subpatent infection primed antigen-specific splenic lymphocytes without inducing lymphocyte apoptosis. Mice were infected with 105 P. c. chabaudi AS pRBC i.v. on day 0. On day 2, the first group of infected mice was killed, along with naive controls (n = 4). Subpatently infected mice were drug-cured on day 2, while patently infected mice were allowed to develop detectable parasitemia. Naive, patently infected, and subpatently infected mice were killed on day 8 (n = 4). Apoptosis of splenic lymphocyte subsets was assessed by staining with annexin V, and proliferation of lymphocytes, stimulated with freeze-thawed P. c. chabaudi AS pRBC was examined on days 2 and 8. Means and SEM are shown. This is representative of two similar experiments.