TABLE 3.

Description of ORFs contained in the BPF genomic island

ORF	Size (bp)		% G + C	Homolog(s)c	Scored	E-valuee
	Aa	Hb
1	501	399	43.1	Phage D108 repressor protein CI	59	3e-08
2	228	219	33.8	Phage D108 Ner DNA-binding protein	80	7e-15
3	2,010	2,061	42.0	HI1478, phage MuA transposase	379	1e-104
4	417	915	42.2	HD0095, phage MuB transposase	156	1e-37
5	204	231	39.7	HD0096, hypothetical protein	47	7e-05
6	507	507	47.1	HI1483, Mu host nuclease inhibitor Gam	186	3e-47
7	159	165	41.5	Haso109501, hypothetical protein	53	7e-07
8	375	465	37.6	Phage PY54 ORF 50	70	4e-12
9	354	276	40.1	HD0495, hypothetical protein	44	0.0005
10	489	462	35.7	HD0110, Mu-like E16 protein	120	1e-26
11	375	351	36.4	CV2165, phage transcriptional regulator	119	1e-26
12	420	NAf	32.6	No significant homology	NA	NA
13	537	519	44.7	HD0112, N-acetylmuramoyl-l-alanine amidase	305	3e-82
14	231	228	39.8	HD0114, conserved hypothetical protein	113	7e-25
15	384	252	38.0	HI1496, hypothetical protein	74	3e-13
16	309	330	37.9	Plu3445, unnamed protein	87	5e-17
17	303	309	41.2	Plu3444, unnamed protein	140	6e-33
18	549	543	40.3	Plu3443, unnamed protein	165	4e-40
19	1,323	1,374	44.6	DRA0094, conserved hypothetical	207	6e-52
20	1,416	1,464	41.2	CV2139, conserved hypothetical	432	1e-120
21	1,278	1,248	38.0	HD1557, bacteriophage Mu GP30-like	211	4e-53
22	189	NA	33.9	No significant homology	NA	NA
23	501	489	41.1	HD1558, bacteriophage Mu G-like	127	8e-29
24	1,104	1,092	43.0	Plu3438, hypothetical protein	289	7e-77
25	927	924	38.5	CV2134, hypothetical protein	209	9e-53
26	318	1,014	38.1	Erp22, B. burgdorferi OM protein	55	2e-07
27	435	447	41.9	Plu3435, hypothetical protein	99	2e-20
28	498	462	42.0	CV2130, hypothetical protein	97	1e-19
29	1,386	1,425	41.2	ORF 1, E. carotovora tail sheath protein	374	1e-102
30	519	522	41.7	ORF 2, E. carotovora tail core protein	147	2e-34
31	285	342	39.3	PA0624, hypothetical protein	43	0.001
32	2,592	2,550	41.3	Plu3429, hypothetical protein	271	7e-71
33	933	882	39.8	STY1630, hypothetical protein	113	5e-24
34	231	213	40.1	STY1631, bacteriophage tail fiber protein	80	1e-14
35	1,065	1,170	39.6	CV2118, bacteriophage regulatory protein	279	7e-74
36	609	402	42.0	Plu3426, phage baseplate component	121	1e-26
37	366	357	39.7	STM4203, phage baseplate protein	74	4e-13
38	1,107	1,101	42.8	STY1635, bacteriophage baseplate protein	256	9e-67
39	570	576	41.8	STY1636, bacteriophage tail fiber protein	145	5e-34
40	1,710	2,775	39.2	HP1p38, phage tail fiber protein	608	1e-172
41	603	630	36.2	HP2p34, phage tail collar	361	5e-99
42	501	456	37.9	Hflu2020135, enoyl-coenzyme A hydratase/carnithine racemase	251	6e-66
43	120	117	34.2	HI1522.1, Mu-like prophage protein Com	74	6e-13
44	846	888	38.5	HI1523, hypothetical protein	412	1e-114

^aActual size of ORF in the F3031 genomic island.

^bSize of the homolog.

^cLetters followed by locus numbers are as dictated by finished sequences. HI represents locus numbers from H. influenzae Rd KW20, Haso from H. somnus 2336, Hflu from H. influenzae R2866, HD from H. ducreyi 35000HP, CV from C. violaceum ATCC 12472, Plu from P. luminescens subsp. laumondii TT01, DR from D. radiodurans R1, PAO from P. aeruginosa PAO1, STM from S. enterica serovar Typhimurium LT2, and STY S. enterica subsp. enterica serovar Typhi CT18.

^dA score of <40 was not considered significant.

^eBlastx analysis using the NCBI website was used to get the expectation value (E-value).

^fNA, not applicable.