TABLE 1.
Procedure | Sp acta | Total activityb | % Yield | Purification factor |
---|---|---|---|---|
Enzyme extract | 6.0 × 10−2 | 20.5 × 10−2 | 100 | 1 |
DEAE-Sepharose | 15.0 × 10−2 | 18.0 × 10−2 | 88 | 3 |
Superose 6 | 102.9 × 10−2 | 15.4 × 10−2 | 73 | 32 |
Specific activity: nanomoles of AMC released per minute per milligram of protein.
Total activity: specific activity × total protein.