TABLE 2.
Identification of B. burgdorferi protease by peptide mass fingerprinting
| Tryptic peptide mass (Da)
|
Identified amino acid sequence | |
|---|---|---|
| Experimental dataa (in-gel digestion) | B. burgdorferi aminopeptidase (in silico digestion) | |
| 671.680 | 671.795 | 16GINLQK21 |
| 1,183.352 | 1,183.261 | 288VVDFGCDDEK297 |
| 1,314.408 | 1,314.414 | 159EGSQTLEEFFK169 |
| 1,919.089 | 1,919.249 | 199TLNELNLEKVIFKTEK214 |
| 2,890.105 | 2,890.220 | 236GTEIEFNANMPTEEVFTTP NYKKTCK260 |
| 1,919.152 | 1,919.249 | 199TLNELNLEKVIFKTEK214 |
| 2,187.480 | 2,187.656 | 39ILAKKAYEHAKYVKLNIK57 |
| 1,200.348 | 1,200.444 | 58DIDILKSRLK67 |
a
Masses are MH+ monoisotopic, with accuracy of 0.1 to 0.2 Da. The enzyme was digested with trypsin, and masses of resulting peptides were determined by electrospray ionization-mass spectrometry and compared to theoretical ones produced by in silico digestion of proteins found in the database (SwissProt).