TABLE 3.
Preincubation temp of enzyme (°C) | Enzymatic activity (% of control)
|
|
---|---|---|
10-min preincubation | 240-min preincubation | |
25 | 100 | 98 |
37 | 101 | 99 |
50 | 105 | 95 |
60 | 102 | 87.5 |
70 | 25 | 9 |
100 | 0 | 0 |
Purified enzyme was preincubated at different temperatures for 10 or 240 min, and then its activity on Leu-AMC was measured at 60°C. Control consisted of enzymatic activity assay without previous incubation of enzyme.