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. 2005 Apr;73(4):2253–2261. doi: 10.1128/IAI.73.4.2253-2261.2005

TABLE 3.

TAPBb is thermostablea

Preincubation temp of enzyme (°C) Enzymatic activity (% of control)
10-min preincubation 240-min preincubation
25 100 98
37 101 99
50 105 95
60 102 87.5
70 25 9
100 0 0
a

Purified enzyme was preincubated at different temperatures for 10 or 240 min, and then its activity on Leu-AMC was measured at 60°C. Control consisted of enzymatic activity assay without previous incubation of enzyme.