FIG. 2.

Cells transfected with pHED-7 exhibit reduced susceptibility to Shiga toxin. Vero cells were transfected with either plasmid alone (WT) or plasmid a containing truncated HEDJ cDNA (pHED-7). Neomycin-resistant cells were selected for 2 weeks and then exposed to Shiga toxin for 4 h. The cells were pulsed with [³⁵S]methionine for 30 min in methionine-free medium. After several washes, the cells were lysed, TCA was added to a concentration of 10%, and the amount of radioactivity incorporated into protein was quantified by scintillation counting of the TCA precipitate. Two asterisks indicate that the difference in [³⁵S]methionine incorporation between untreated and toxin-treated wild-type cells is statistically significant (P < 0.05).