FIG. 6.
StxA is found in a complex that includes HEDJ and Sec61. StxA was synthesized by coupled in vitro transcription and translation in the presence of 35S-labeled methionine and cysteine and ER extracts as described previously. After cross-linking with DSP, the first immunoprecipitation was carried out with antibodies against HEDJ. The immunoprecipitated material was heated to 95°C for 5 min and then subjected to a second round of immunoprecipitation with either no second antibody (Ab) or antibody against Sec61, BiP, or Grp94.