TABLE 2.
Cytokine gene expression in AILC and EILC of R- and S-line sheep prior to experimental infection with T. colubriformis
| Cytokine | ΔCT (mean ± SE)a
|
|||
|---|---|---|---|---|
| R-line sheep
|
S-line sheep
|
|||
| AILC (n = 5) | EILC (n = 4) | AILC (n = 3) | EILC (n = 6) | |
| IL-4 | 10.1 ± 0.76 | 11.0 ± 0.96 | 8.9 ± 1.02 | 11.4 ± 0.68 |
| IL-5 | 12.3 ± 1.92 | 15.7 ± 2.43 | 16.1 ± 2.57 | 15.8 ± 1.72 |
| IL-10 | 7.6 ± 1.22 | 11.1 ± 1.54 | 9.3 ± 1.63 | 11.9 ± 1.09 |
| IL-13 | 10.8 ± 1.57 | 10.1 ± 1.98 | 13.0 ± 2.10 | 12.1 ± 1.40 |
| IFN-γ | 9.7 ± 1.53 | 8.7 ± 1.94 | 7.3 ± 2.05 | 9.3 ± 1.37 |
| TNF-α | 5.8 ± 0.94 | 7.2 ± 1.18 | 6.2 ± 1.26 | 7.3 ± 0.84 |
a
Values were derived from lymph samples collected over the 3 days immediately preceding challenge. ΔCT represents the difference in threshold cycles between the target and house keeping genes. Therefore, low ΔCT values represent high gene expression levels and high ΔCT values represent low gene expression levels. n, number of sheep in group.