TABLE 3.

Mean ΔC_T and mean slope of cytokine gene expression in AILC and EILC of R- and S-line sheep during experimental infection with T. colubriformis^a

Cytokine	Value for R-line sheep (n = 9)		Value for S-line sheep (n = 9)
	Mean ΔCT ± SE	Mean slope ± SE	Mean ΔCT ± SE	Mean slope ± SE
IL-5	13.6 ± 1.06b	−0.07 ± 0.029	16.7 ± 1.06	0.03 ± 0.029
IL-13	9.0 ± 0.69b	−0.16 ± 0.034c	12.8 ± 0.69	0.03 ± 0.034
TNF-αd	7.3 ± 0.53	0.00 ± 0.023	8.0 ± 0.53	0.10 ± 0.230
IL-10	10.3 ± 0.50	0.01 ± 0.032	10.5 ± 0.47	−0.01 ± 0.031
IFN-γ	9.7 ± 0.78	−0.03 ± 0.022	8.7 ± 0.78	0.01 ± 0.022
IL-4	10.5 ± 0.44	0.00 ± 0.019	11.0 ± 0.44	0.04 ± 0.019

^aFor each animal samples were collected daily over a period of 18 to 21 days. The lymphatic compartment and the preinfection status of the animals were not taken into consideration. ΔC_T represents the difference in threshold cycles for the target and housekeeping genes. Therefore, low ΔC_T values represent high gene expression levels and high ΔC_T values represent low gene expression levels.

^bSignificantly (P < 0.05 for IL-5; P < 0.01 for IL-13) higher expression in R-line than S-line animals during T. colubriformis infection.

^cSignificant (P < 0.01) trend for up-regulation of the IL-13 gene in R-line sheep during infection with T. colubriformis.

^dSignificant (P < 0.01) difference in the trend for TNF-α gene expression between R- and S-line animals during T. colubriformis infection.