TABLE 6.
Expression of cell differentiation markers on leucocytes in afferent and efferent intestinal lymph cells after experimental infection with T. colubriformis
| Marker | % of cells positive (mean ± SDM) at:a
|
||||
|---|---|---|---|---|---|
| Time 0 | Week 1 | Week 2 | Week 3 | Week 4 | |
| Marker in EILC | |||||
| CD4 | 51.5 ± 11.3 | 43.5 ± 8.4b | 37.5 ± 9.4b | 38.1 ± 7.0b | 37.6 ± 4.4b |
| CD8 | 31.4 ± 12.6 | 29.4 ± 7.9 | 24.7 ± 5.1 | 23.4 ± 5.2 | 26.0 ± 5.4 |
| γδ TCR | 5.6 ± 2.0 | 6.4 ± 2.0 | 7.9 ± 4.1 | 8.0 ± 2.6 | 9.3 ± 5.6 |
| CD21 | 20.4 ± 11.9 | 19.3 ± 10.0 | 15.4 ± 7.5 | 16.6 ± 7.0 | 15.3 ± 6.3 |
| Marker in AILC | |||||
| CD4 | 54.5 ± 9.6 | 55.9 ± 8.5 | 54.1 ± 8.7 | 53.8 ± 8.0 | NT |
| CD8 | 29.0 ± 9.2 | 29.4 ± 5.0 | 28.3 ± 7.5 | 21.8 ± 7.8 | NT |
| γδ TCR | 3.9 ± 1.4 | 5.1 ± 2.5 | 5.7 ± 3.8 | 6.9 ± 3.1 | NT |
| CD21 | 8.9 ± 6.5 | 10.6 ± 4.1 | 10.0 ± 5.1 | 11.8 ± 5.4 | NT |
| MHC-II | 34.3 ± 8.3 | 37.2 ± 9.0 | 43.0 ± 11.4 | 40.4 ± 11.7 | NT |
| CD11c | 11.2 ± 3.2 | 15.0 ± 1.1 | 14.7 ± 2.1 | 14.8 ± 3.4 | NT |
| CD1b | 1.5 ± 1.0 | 3.0 ± 1.6 | 2.6 ± 0.8 | 2.4 ± 0.1 | NT |
| CD25 | 10.8 ± 4.2 | 12.8 ± 3.5 | 11.3 ± 5.3 | 15.1 ± 7.5 | NT |
a
Data from R-line and S-line sheep have been combined, since no genotype-related differences in cell subset frequencies occurred after experimental infection (see text). For EILC, n = 10 to 11; for AILC, n = 6 to 10. NT, not tested.
b
Values different from time zero (P < 0.05).