TABLE 2.
Analysis of attenuated strains of M. bovis produced by signature tag mutagenesisa
| Mutant strain | Size of deletion (bp) | Gene(s) disrupted or deleted | Gene information | No. of animals with lesions in:
|
Total no. of spleen lesions in 3 animals | |
|---|---|---|---|---|---|---|
| Spleen | Other organs | |||||
| WAg539 | 10,141 | Mb0453c-Mb0461 | mmpL4, mmpS4 (membrane proteins), ufaA1 (lipid, metabolism), sigK (σ factor) and unknown function | 0/3 | 0/3 | 0 |
| WAg567 | 14,310 | Mb3908-Mb3918c | Genes downstream of esxA | 0/3 | 0/3 | 0 |
| WAg568 | 3 | Mb0345c | Unknown function, upstream of aspC | 0/3 | 0/3 | 0 |
| WAg585 | 13,941 | Mb0198-Mb0207c | Unknown function | 0/3 | 0/3 | 0 |
| WAg570 | 0 | Mb3575c | Unknown function, immediately upstream of fadE28 | 1/3 | 0/3 | 1 |
| WAg572 | 34 | Mb3900 | Gene upstream of esxA | 1/3 | 0/3 | 2 |
| WAg571 | 132 | Mb2960-Mb2961 | ppsE (phenolphthiocerol synthesis) and drrA (daunorubicin resistance) | 1/3 | 0/3 | 3 |
| WAg573 | 6,876 | Mb3898-Mb3903 | Genes upstream of esxA | 2/3 | 0/3 | 5 |
| WAg574 | 5,906 | Mb3899-Mb3902 | Genes upstream of esxA | 2/3 | 0/3 | 8 |
| WAg579 | 2 | Mb2971c | pks1 (polyketide synthase) | 3/3 | 0/3 | 38 |
| WAg576 | 2,318 | Mb3897-Mb3899 | Genes upstream of esxA | 3/3 | 0/3 | 50 |
| WAg578 | 2,337 | Mb3905-Mb3907 | esxA and two downstream genes | 2/3 | 1/3 | 51 |
| WAg575 | 145 | Mb2280c | Unknown function | 2/3 | 0/3 | 52 |
| WAg540 | 33 | Mb3901 | Gene upstream of esxA | 1/3 | 1/3 | 100 |
| WAg577 | 7,678 | Mb1848-Mb1852 | secA2 (protein translocase), ilvG (acetolactate synthase), PE_PGRS, and unknown function | 3/3 | 0/3 | 125 |
| Wild type | 3/3 | 3/3 | >300 | |||
| WAg756ko | 574 | Mb3904-Mb3906 | Allelic-exchange knockout of esxA | 1/3 | 0/3 | 10 |
a
Mutants with interrupted genes at or near esxA are shown in boldface.