FIG. 4.
Effect of NK cell depletion on PFC responses. Splenocytes for saline-inoculated (normal) and SL3235-inoculated (immune) mice were isolated 7 days postinoculation. The cells were treated with asialo GM1 antiserum (α) plus rabbit complement to deplete NK cells, as described in Materials and Methods, or left untreated and cultured for 5 days with sheep erythrocytes. Selected wells were treated with IFN-γ (100 U/ml). PFC responses were assessed as described in Materials and Methods. The experiment was done three times. Data are means ± standard deviations of triplicate samples from a representative experiment.