FIG. 2.

Lack of lipid modification of lipoproteins in the lgt mutant. Coomassie blue staining of membrane proteins (A) and autoradiograph of membrane proteins in the same gel (B). Wild-type S. aureus, the lgt mutant, and the complemented mutant were cultivated in MHB medium with shaking for 4.5 h at 37°C. Proteins were labeled and isolated as described in Materials and Methods and separated by SDS-PAGE on 12.5% polyacrylamide gels. Right margin, size markers in kilodaltons.