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Cell-wall-associated proteins. Cell-wall-associated lipoproteins were released from whole bacterial cells with 1.5 M LiCl. Bacteria were grown in MHB supplemented with [14C]palmitic acid. Total protein (12 μg) was separated on an SDS-12.5% polyacrylamide gel and stained with Coomassie brilliant blue (A and B). Gels were dried and autoradiographed for 4 or 5 days (C). WT, wild type.
