FIG. 4.

Release of proteins into the culture supernatant. Proteins from culture supernatants of stationary phase cultures (grown in BM for 14 h with shaking) of S. aureus wild type, the lgt mutant, and the complemented mutant were separated by SDS-PAGE and stained with Coomassie brilliant blue. At least 14 additional or more highly expressed protein bands (arrows) were visually detected in the supernatant of the lgt mutant. Some of the proteins were cut out and partially sequenced. Autolysin (Atl), oligopeptide-binding protein (OppA) and autolysin homolog protein comigrate; an unidentified protein and 1 to 4 homologous proteins comigrate at the 35-kDa position. PrsA, peptidyl-prolyl cis-trans isomerase; SitC, binding protein of staphylococcal iron transporter.