Figure 4.

Proteome comparison of Prdm16^csp1/wt cardiac tissue. Volcano plots for Prdm16^csp1/wt/control pairwise comparisons with log2 ratio (x-axis) and P-value (y-axis) of male hearts (A), female hearts (B), and combined analysis of male + female hearts (C). Colouring indicates significantly altered proteins (threshold dashed grey line, P-value < 0.01), and * highlights highly significantly different proteins with P-value < 0.0001. (D) Heat map of all regulated proteins (P-value < 0.01, abs log2 ratio > 0.5) using z-scored values of group median intensity. Pre-B-cell leukaemia transcription factor interacting protein 1 (Pbxip1) and pyridine nucleotide-disulphide oxidoreductase domain 2 (Pyroxd2) are consistently increased in male and female Prdm16^csp1/wt mice with P-value < 0.0001. Analysed biological replicates are n = 4 in each group. (E) Western blot analysis shows protein expression of selected mitochondrial proteins. (F) Quantification of western blot data showing mitochondrial protein levels. Analysed biological replicates are n > 4. Statistical analysis was performed with unpaired t-test, * indicates P < 0.05.